Timberlake inducted Madonna to the Rock and Roll Hall of Fame yesterday (March 10), and during his speech made reference to Spears.
The singer told the audience, "The world has always been full of Madonna wannabes and I might have even dated a couple."
"She has still found time to kiss someone I may or may not have publicly kissed myself while I was in the audience. Of course you know I was talking about Sean Penn," added Timberlake.
Madonna accepted the honour by thanking her family and fans who have helped her career.