A source told People that the celebrity couple, who seemed to be getting along fine at the last Oscars ceremony, are no more.
After the actress returned from her trip to Europe to promote her film Marley & Me, the lovebirds decided to call it quits.
"They had some disagreements and decided to not continue to see each other. Jen is moving on with her life like she always does. She seems happy," the source added.
This isn’t the first time Mayer and Aniston have announced a separation. In August 2008, the 31-year-old singer told the press that he broke up with the actress because he didn’t think she was the one for him.