The Universal Pictures project follows the story of two heterosexual firefighters (Adam Sandler and Kevin James) who pose as a gay couple to qualify for the rights and advantages of a married couple.
Buscemi will play a city official who is sent to keep and eye on Chuck and Larry and is determined to reveal the truth. Aykroyd will play the fire captain.
Dennis Dugan will direct the project, which also stars Jessica Biel.